論文

査読有り
2018年9月

Metastatic tumor cells detection and anti-metastatic potential with vesicular stomatitis virus in immunocompetent murine model of osteosarcoma

JOURNAL OF ORTHOPAEDIC RESEARCH
  • Johan, Muhammad P
  • ,
  • Kubo, Tadahiko
  • ,
  • Furuta, Taisuke
  • ,
  • Sakuda, Tomohiko
  • ,
  • Sakaguchi, Takemasa
  • ,
  • Nakanishi, Mahito
  • ,
  • Ochi, Mitsuo
  • ,
  • Adachi, Nobuo

36
9
開始ページ
2562
終了ページ
2569
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/jor.23911
出版者・発行元
WILEY

Sarcomas are associated with a high incidence of lung metastasis, which leads to a high-risk of cancer death. This study was performed to explore the pre-clinical theranostic potential of a novel fully functional recombinant vesicular stomatitis virus carrying imaging gene Katushka (rVSV-K), as virotherapy and circulating tumor cells (CTCs) detection in the syngeneic mouse model of osteosarcoma with spontaneous pulmonary metastases. Recombinant VSV-K was generated and evaluated in vitro on human and murine osteosarcoma cells. Spontaneous osteosarcoma metastases were established in immune-competent mice by implanting subcutaneously syngeneic osteosarcoma LM8 cells. The vector was injected into the tumor-bearing mice via jugular vein either once or repeatedly. To assess effectiveness, primary tumor growth and development of lung metastasis as well as survival were evaluated. We found that rVSV-K efficiently replicated in and killed all osteosarcoma cell lines in time-dependent manners. Both single or repeated systemic injections of the virus did not inhibit the growth of the primary tumor, but the repeated administration could effectively suppress the development of lung metastases a

リンク情報
DOI
https://doi.org/10.1002/jor.23911