論文

査読有り
2015年5月

Casein Kinase 1 gamma Ensures Monopolar Growth Polarity under Incomplete DNA Replication Downstream of Cds1 and Calcineurin in Fission Yeast

MOLECULAR AND CELLULAR BIOLOGY
  • Takayuki Koyano
  • ,
  • Manabu Konishi
  • ,
  • Sophie G. Martin
  • ,
  • Yoshikazu Ohya
  • ,
  • Dai Hirata
  • ,
  • Takashi Toda
  • ,
  • Kazunori Kume

35
9
開始ページ
1533
終了ページ
1542
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1128/MCB.01465-14
出版者・発行元
AMER SOC MICROBIOLOGY

Cell polarity is essential for various cellular functions during both proliferative and developmental stages, and it displays dynamic alterations in response to intracellular and extracellular cues. However, the molecular mechanisms underlying spatiotemporal control of polarity transition are poorly understood. Here, we show that fission yeast Cki3 (a casein kinase 1 gamma homolog) is a critical regulator to ensure persistent monopolar growth during S phase. Unlike the wild type, cki3 mutant cells undergo bipolar growth when S phase is blocked, a condition known to delay transition from monopolar to bipolar growth (termed NETO [new end takeoff]). Consistent with this role, Cki3 kinase activity is substantially increased, and cells lose their viability in the absence of Cki3 upon an S-phase block. Cki3 acts downstream of the checkpoint kinase Cds1/Chk2 and calcineurin, and the latter physically interacts with Cki3. Autophosphorylation in the C terminus is inhibitory toward Cki3 kinase activity, and calcineurin is responsible for its dephosphorylation. Cki3 localizes to the plasma membrane, and this localization requires the palmitoyltransferase complex Erf2-Erf4. Membrane localization is needed not only for proper NETO timing but also for Cki3 kinase activity. We propose that Cki3 acts as a critical inhibitor of cell polarity transition under S-phase arrest.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1128/MCB.01465-14
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000352280900005&DestApp=WOS_CPL
ID情報
  • DOI : 10.1128/MCB.01465-14
  • ISSN : 0270-7306
  • eISSN : 1098-5549
  • Web of Science ID : WOS:000352280900005

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