論文

査読有り
2015年5月

Screening for a gene deletion mutant whose temperature sensitivity is suppressed by FK506 in budding yeast and its application for a positive screening for drugs inhibiting calcineurin

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
  • Kazunori Kume
  • ,
  • Takayuki Koyano
  • ,
  • Junpei Takata
  • ,
  • Ko Wakabayashi
  • ,
  • Masaki Mizunuma
  • ,
  • Tokichi Miyakawa
  • ,
  • Dai Hirata

79
5
開始ページ
790
終了ページ
794
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/09168451.2014.1003132
出版者・発行元
TAYLOR & FRANCIS LTD

Calcineurin, which is a Ca2+/calmodulin-dependent protein phosphatase, is a key mediator in calcium signaling in diverse biological processes and of clinical importance as the target of the immunosuppressant FK506. To identify a mutant(s) in which calcineurin is activated, inhibiting cellular growth as a result, we screened for a mutant(s) whose temperature sensitivity would be suppressed by FK506 from the budding yeast non-essential gene deletion library. We found that the temperature sensitivity of cells in which the conserved Verprolin VRP1 gene had been deleted, which gene is required for actin organization and endocytosis, was suppressed by either FK506 or by cnb1 deletion. Indeed, the calcineurin activity increased significantly in the increment vrp1 cells. Finally, we demonstrated that the increment vrp1 strain to be useful as an indicator in a positive screening for bioactive compounds inhibiting calcineurin.

Web of Science ® 被引用回数 : 1

リンク情報
DOI
https://doi.org/10.1080/09168451.2014.1003132
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000354463700014&DestApp=WOS_CPL
ID情報
  • DOI : 10.1080/09168451.2014.1003132
  • ISSN : 0916-8451
  • eISSN : 1347-6947
  • Web of Science ID : WOS:000354463700014

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