論文

査読有り
2015年7月

Isolation of a spontaneous cerulenin-resistant sake yeast with both high ethyl caproate-producing ability and normal checkpoint integrity

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
  • Hiroyasu Tamura
  • ,
  • Hiroki Okada
  • ,
  • Kazunori Kume
  • ,
  • Takayuki Koyano
  • ,
  • Tetsuya Goshima
  • ,
  • Ryo Nakamura
  • ,
  • Takeshi Akao
  • ,
  • Hitoshi Shimoi
  • ,
  • Masaki Mizunuma
  • ,
  • Yoshikazu Ohya
  • ,
  • Dai Hirata

79
7
開始ページ
1191
終了ページ
1199
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/09168451.2015.1020756
出版者・発行元
TAYLOR & FRANCIS LTD

In the brewing of high-quality sake such as Daiginjo-shu, the cerulenin-resistant sake yeast strains with high producing ability to the flavor component ethyl caproate have been used widely. Genetic stability of sake yeast would be important for the maintenance of both fermentation properties of yeast and quality of sake. In eukaryotes, checkpoint mechanisms ensure genetic stability. However, the integrity of these mechanisms in sake yeast has not been examined yet. Here, we investigated the checkpoint integrity of sake yeasts, and the results suggested that a currently used cerulenin-resistant sake yeast had a defect in spindle assembly checkpoint (SAC). We also isolated a spontaneous cerulenin-resistant sake yeast FAS2-G1250S mutant, G9CR, which showed both high ethyl caproate-producing ability and integrity/intactness of the checkpoint mechanisms. Further, morphological phenotypic robustness analysis by use of CalMorph supported the genetic stability of G9CR. Finally, we confirmed the high quality of sake from G9CR in an industrial sake brewing setting.

Web of Science ® 被引用回数 : 11

リンク情報
DOI
https://doi.org/10.1080/09168451.2015.1020756
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000357330200025&DestApp=WOS_CPL
ID情報
  • DOI : 10.1080/09168451.2015.1020756
  • ISSN : 0916-8451
  • eISSN : 1347-6947
  • Web of Science ID : WOS:000357330200025

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