2015年9月
The essential function of Rrs1 in ribosome biogenesis is conserved in budding and fission yeasts
YEAST
- 巻
- 32
- 号
- 9
- 開始ページ
- 607
- 終了ページ
- 614
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/yea.3083
- 出版者・発行元
- WILEY
The Rrs1 protein plays an essential role in the biogenesis of 60S ribosomal subunits in budding yeast (Saccharomyces cerevisiae). Here, we examined whether the fission yeast (Schizosaccharomyces pombe) homologue of Rrs1 also plays a role in ribosome biogenesis. To this end, we constructed two temperature-sensitive fission yeast strains, rrs1-D14/22G and rrs1-L51P, which had amino acid substitutions corresponding to those of the previously characterized budding yeast rrs1-84 (D22/30G) and rrs1-124 (L61P) strains, respectively. The fission yeast mutants exhibited severe defects in growth and 60S ribosomal subunit biogenesis at high temperatures. In addition, expression of the Rrs1 protein of fission yeast suppressed the growth defects of the budding yeast rrs1 mutants at high temperatures. Yeast two-hybrid analyses revealed that the interactions of Rrs1 with the Rfp2 and Ebp2 proteins were conserved in budding and fission yeasts. These results suggest that the essential function of Rrs1 in ribosome biogenesis may be conserved in budding and fission yeasts. Copyright (c) 2015 John Wiley & Sons, Ltd.
- リンク情報
- ID情報
-
- DOI : 10.1002/yea.3083
- ISSN : 0749-503X
- eISSN : 1097-0061
- Web of Science ID : WOS:000360981300003