論文

査読有り
2015年12月

Casein kinase 1 gamma acts as a molecular switch for cell polarization through phosphorylation of the polarity factor Tea1 in fission yeast

GENES TO CELLS
  • Takayuki Koyano
  • ,
  • Karin Barnouin
  • ,
  • Ambrosius P. Snijders
  • ,
  • Kazunori Kume
  • ,
  • Dai Hirata
  • ,
  • Takashi Toda

20
12
開始ページ
1046
終了ページ
1058
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/gtc.12309
出版者・発行元
WILEY

Fission yeast undergoes growth polarity transition from monopolar to bipolar during G2 phase, designated NETO (New End Take Off). It is known that NETO onset involves two prerequisites, the completion of DNA replication and attainment of a certain cell size. However, the molecular mechanism remains unexplored. Here, we show that casein kinase 1 gamma, Cki3 is a critical determinant of NETO onset. Not only did cki3 Delta cells undergo NETO during G1- or S-phase, but they also displayed premature NETO under unperturbed conditions with a smaller cell size, leading to cell integrity defects. Cki3 interacted with the polarity factor Tea1, of which phosphorylation was dependent on Cki3 kinase activity. GFP nanotrap of Tea1 by Cki3 led to Tea1 hyperphosphorylation with monopolar growth, whereas the same entrapment by kinase-dead Cki3 resulted in converse bipolar growth. Intriguingly, the Tea1 interactor Tea4 was dissociated from Tea1 by Cki3 entrapment. Mass spectrometry identified four phosphoserine residues within Tea1 that were hypophosphorylated in cki3 Delta cells. Phosphomimetic Tea1 mutants showed compromised binding to Tea4 and NETO defects, indicating that these serine residues are critical for protein-protein interaction and NETO onset. Our findings provide significant insight into the mechanism by which cell polarization is regulated in a spatiotemporal manner.

Web of Science ® 被引用回数 : 3

リンク情報
DOI
https://doi.org/10.1111/gtc.12309
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000368258600008&DestApp=WOS_CPL
ID情報
  • DOI : 10.1111/gtc.12309
  • ISSN : 1356-9597
  • eISSN : 1365-2443
  • Web of Science ID : WOS:000368258600008

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