論文

査読有り
2016年8月

Identification of a mutation causing a defective spindle assembly checkpoint in high ethyl caproate-producing sake yeast strain K1801

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
  • Tetsuya Goshima
  • ,
  • Ryo Nakamura
  • ,
  • Kazunori Kume
  • ,
  • Hiroki Okada
  • ,
  • Eri Ichikawa
  • ,
  • Hiroyasu Tamura
  • ,
  • Hirokazu Hasuda
  • ,
  • Masaaki Inahashi
  • ,
  • Naoto Okazaki
  • ,
  • Takeshi Akao
  • ,
  • Hitoshi Shimoi
  • ,
  • Masaki Mizunuma
  • ,
  • Yoshikazu Ohya
  • ,
  • Dai Hirata

80
8
開始ページ
1657
終了ページ
1662
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/09168451.2016.1184963
出版者・発行元
TAYLOR & FRANCIS LTD

In high-quality sake brewing, the cerulenin-resistant sake yeast K1801 with high ethyl caproate-producing ability has been used widely; however, K1801 has a defective spindle assembly checkpoint (SAC). To identify the mutation causing this defect, we first searched for sake yeasts with a SAC-defect like K1801 and found that K13 had such a defect. Then, we searched for a common SNP in only K1801 and K13 by examining 15 checkpoint-related genes in 23 sake yeasts, and found 1 mutation, R48P of Cdc55, the PP2A regulatory B subunit that is important for the SAC. Furthermore, we confirmed that the Cdc55-R48P mutation was responsible for the SAC-defect in K1801 by molecular genetic analyses. Morphological analysis indicated that this mutation caused a high cell morphological variation. But this mutation did not affect the excellent brewing properties of K1801. Thus, this mutation is a target for breeding of a new risk-free K1801 with normal checkpoint integrity.

Web of Science ® 被引用回数 : 11

リンク情報
DOI
https://doi.org/10.1080/09168451.2016.1184963
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000379673000030&DestApp=WOS_CPL
ID情報
  • DOI : 10.1080/09168451.2016.1184963
  • ISSN : 0916-8451
  • eISSN : 1347-6947
  • Web of Science ID : WOS:000379673000030

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