論文

査読有り
2017年5月

A systematic genomic screen implicates nucleocytoplasmic transport and membrane growth in nuclear size control

PLOS GENETICS
  • Kazunori Kume
  • ,
  • Helena Cantwell
  • ,
  • Frank R. Neumann
  • ,
  • Andrew W. Jones
  • ,
  • Ambrosius P. Snijders
  • ,
  • Paul Nurse

13
5
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pgen.1006767
出版者・発行元
PUBLIC LIBRARY SCIENCE

How cells control the overall size and growth of membrane-bound organelles is an important unanswered question of cell biology. Fission yeast cells maintain a nuclear size proportional to cellular size, resulting in a constant ratio between nuclear and cellular volumes (N/C ratio). We have conducted a genome-wide visual screen of a fission yeast gene deletion collection for viable mutants altered in their N/C ratio, and have found that defects in both nucleocytoplasmic mRNA transport and lipid synthesis alter the N/C ratio. Perturbing nuclear mRNA export results in accumulation of both mRNA and protein within the nucleus, and leads to an increase in the N/C ratio which is dependent on new membrane synthesis. Disruption of lipid synthesis dysregulates nuclear membrane growth and results in an enlarged N/C ratio. We propose that both properly regulated nucleocytoplasmic transport and nuclear membrane growth are central to the control of nuclear growth and size.

Web of Science ® 被引用回数 : 19

リンク情報
DOI
https://doi.org/10.1371/journal.pgen.1006767
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000402884800020&DestApp=WOS_CPL
ID情報
  • DOI : 10.1371/journal.pgen.1006767
  • ISSN : 1553-7404
  • Web of Science ID : WOS:000402884800020

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