論文

査読有り
2016年1月

Stomach Chitinase from Japanese Sardine Sardinops melanostictus: Purification, Characterization, and Molecular Cloning of Chitinase Isozymes with a Long Linker

MARINE DRUGS
  • Satoshi Kawashima
  • ,
  • Hiroki Ikehata
  • ,
  • Chihiro Tada
  • ,
  • Tomohiro Ogino
  • ,
  • Hiromi Kakizaki
  • ,
  • Mana Ikeda
  • ,
  • Hideto Fukushima
  • ,
  • Masahiro Matsumiya

14
1
開始ページ
1
終了ページ
13
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/md14010022
出版者・発行元
MDPI AG

Fish express two different chitinases, acidic fish chitinase-1 (AFCase-1) and acidic fish chitinase-2 (AFCase-2), in the stomach. AFCase-1 and AFCase-2 have different degradation patterns, as fish efficiently degrade chitin ingested as food. For a comparison with the enzymatic properties and the primary structures of chitinase isozymes obtained previously from the stomach of demersal fish, in this study, we purified chitinase isozymes from the stomach of Japanese sardine Sardinops melanostictus, a surface fish that feeds on plankton, characterized the properties of these isozymes, and cloned the cDNAs encoding chitinases. We also predicted 3D structure models using the primary structures of S. melanostictus stomach chitinases. Two chitinase isozymes, SmeChiA (45 kDa) and SmeChiB (56 kDa), were purified from the stomach of S. melanostictus. Moreover, two cDNAs, SmeChi-1 encoding SmeChiA, and SmeChi-2 encoding SmeChiB were cloned. The linker regions of the deduced amino acid sequences of SmeChi-1 and SmeChi-2 (SmeChi-1 and SmeChi-2) are the longest among the fish stomach chitinases. In the cleavage pattern groups toward short substrates and the phylogenetic tree analysis, SmeChi-1 and SmeChi-2 were classified into AFCase-1 and AFCase-2, respectively. SmeChi-1 and SmeChi-2 had catalytic domains that consisted of a TIM-barrel (beta/alpha)(8)-fold structure and a deep substrate-binding cleft. This is the first study showing the 3D structure models of fish stomach chitinases.

リンク情報
DOI
https://doi.org/10.3390/md14010022
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000374586800021&DestApp=WOS_CPL
ID情報
  • DOI : 10.3390/md14010022
  • ISSN : 1660-3397
  • Web of Science ID : WOS:000374586800021

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