論文

査読有り 国際誌
2020年9月

Reliability of serological tests for COVID-19: comparison of three immunochromatography test kits for SARS-CoV-2 antibodies

Heliyon
  • Hidetsugu Fujigaki
  • Masao Takemura
  • Michiko Osawa
  • Aki Sakurai
  • Kentaro Nakamoto
  • Koichi Seto
  • Takashi Fujita
  • Tadayoshi Hata
  • Hidehiko Akiyama
  • Yohei Doi
  • Kuniaki Saito
  • 全て表示

6
9
開始ページ
e04929
終了ページ
e04929
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.heliyon.2020.e04929
出版者・発行元
Elsevier BV

Background: Several immunochromatographic serological test kits have been developed to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific antibodies, but their relative performance and potential clinical utility is unclear. Methods: Three commercially available serological test kits were evaluated using 99 serum samples collected from 29 patients diagnosed with coronavirus disease 2019 (COVID-19) and 100 serum samples collected from 100 healthy volunteers in 2017 as negative controls. Results: The specificity of the IgM and IgG antibodies showed comparable results among the three immunochromatographic serological test kits. The specificity for IgM antibody was 98.0%, 98.0%, and 97.0%, and the specificity for IgG antibody was identical among the three kits (99.0%). The IgM antibody-positive rates of the three test kits for samples taken at the early stage of the disease (0-4 days after onset) were consistent with all three kits (18.2%); however, the IgM antibody-positive rates thereafter showed considerable differences among the kits, making it difficult to interpret the kinetics of IgM response against SARS-CoV-2. The IgG antibody-positive rates for samples taken after 13 days of onset were 100.0%, 97.6%, and 97.6%, respectively. Conclusion: There were large differences among the results of the three test kits. Only few cases showed positive results for IgM, suggesting that at least 2 of these kits used in this study were unsuitable for diagnosis of COVID-19. The IgG antibody was positive in almost all samples after 13 days of onset, suggesting that it may be useful for determining infections in the recent past.

リンク情報
DOI
https://doi.org/10.1016/j.heliyon.2020.e04929
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32984615
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7495099
ID情報
  • DOI : 10.1016/j.heliyon.2020.e04929
  • ISSN : 2405-8440
  • PubMed ID : 32984615
  • PubMed Central 記事ID : PMC7495099

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