2017年9月1日
Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA
Journal of Synchrotron Radiation
- 巻
- 24
- 号
- 5
- 開始ページ
- 1086
- 終了ページ
- 1091
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1107/S160057751701030X
- 出版者・発行元
- International Union of Crystallography
X-ray free-electron lasers (XFELs) have opened new opportunities for time-resolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TR-SFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom Compact free-electron LAser (SACLA) is reported. The optical-fiber-based system is a good choice for a quick setup in a limited beam time and allows pump illumination from two directions to achieve high excitation efficiency of protein microcrystals. Two types of injectors are used: one for extruding highly viscous samples such as lipidic cubic phase (LCP) and the other for pulsed liquid droplets. Under standard sample flow conditions from the viscous-sample injector, delay times from nanoseconds to tens of milliseconds are accessible, typical time scales required to study large protein conformational changes. A first demonstration of a TR-SFX experiment on bacteriorhodopsin in bicelle using a setup with a droplet-type injector is also presented.A nanosecond pump-probe device for time-resolved serial femtosecond crystallography has been developed at SACLA.
- リンク情報
- ID情報
-
- DOI : 10.1107/S160057751701030X
- ISSN : 1600-5775
- ISSN : 0909-0495
- SCOPUS ID : 85028730027