MISC

2017年9月1日

Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA

Journal of Synchrotron Radiation
  • Minoru Kubo
  • Eriko Nango
  • Kensuke Tono
  • Tetsunari Kimura
  • Shigeki Owada
  • Changyong Song
  • Fumitaka Mafuné
  • Ken Miyajima
  • Yoshihiro Takeda
  • Jun-Ya Kohno
  • Naoya Miyauchi
  • Takanori Nakane
  • Tomoyuki Tanaka
  • Takashi Nomura
  • Jan Davidsson
  • Rie Tanaka
  • Michio Murata
  • Takashi Kameshima
  • Takaki Hatsui
  • Yasumasa Joti
  • Richard Neutze
  • Makina Yabashi
  • So Iwata
  • 全て表示

24
5
開始ページ
1086
終了ページ
1091
記述言語
英語
掲載種別
DOI
10.1107/S160057751701030X
出版者・発行元
International Union of Crystallography

X-ray free-electron lasers (XFELs) have opened new opportunities for time-resolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TR-SFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom Compact free-electron LAser (SACLA) is reported. The optical-fiber-based system is a good choice for a quick setup in a limited beam time and allows pump illumination from two directions to achieve high excitation efficiency of protein microcrystals. Two types of injectors are used: one for extruding highly viscous samples such as lipidic cubic phase (LCP) and the other for pulsed liquid droplets. Under standard sample flow conditions from the viscous-sample injector, delay times from nanoseconds to tens of milliseconds are accessible, typical time scales required to study large protein conformational changes. A first demonstration of a TR-SFX experiment on bacteriorhodopsin in bicelle using a setup with a droplet-type injector is also presented.A nanosecond pump-probe device for time-resolved serial femtosecond crystallography has been developed at SACLA.

リンク情報
DOI
https://doi.org/10.1107/S160057751701030X
URL
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85028730027&origin=inward
ID情報
  • DOI : 10.1107/S160057751701030X
  • ISSN : 1600-5775
  • ISSN : 0909-0495
  • SCOPUS ID : 85028730027

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