講演・口頭発表等

招待有り 国際会議
2020年1月11日

Modification of  Agrobacterium tumefaciens for Improving the Transformation Frequency in Crops

Plant & Animal Genome Conference 2020
  • Satoko, Nonaka
  • ,
  • Hiroshi, Ezura

記述言語
英語
会議種別
口頭発表(招待・特別)
開催地
United states of America San Diego, California

Agrobacterium tumefaciens has a unique ability to mediate inter-kingdom DNA transfer. This ability of A. tumefaciens has been exploited in fields such as functional analysis of genes and breeding of Generic Modified (GM) crops. However, in some plants, transformation frequency is still low, which creates a technical problem. To overcome the problem, we focused on the transformation process comprising the following three steps: the first step is T-DNA transfer, second is transgenic cell selection, and third is transgenic cell regeneration.
To improve T-DNA transfer frequency, we tried to modify A. tumefaciens, focusing on the T-DNA transfer, which is the first step of transformation process. Several studies showed that ethylene, phytohormone, and gamma aminobutyric acid (GABA), a non-proteomic amino acid, are factors negatively affecting plant–Agrobacterium interactions. Thus, we hypothesized that the removal of these factors would be effective in improving the T-DNA transfer frequency. Based on this hypothesis, four types of Super-Agrobacterium were created and analyzed.
Super-Agrobacterium ver. 1: the target was ethylene. We tried to introduce the 1-aminocyclopropane-1-carboxy