論文

査読有り
2019年6月

Agroinfiltration-based efficient transient protein expression in leguminous plants

Plant Biotechnology
  • Suzaki, Takuya
  • ,
  • Tsuda, Mai
  • ,
  • Ezura, Hiroshi
  • ,
  • Day, Brad
  • ,
  • Miura, Kenji

36
2
開始ページ
119
終了ページ
123
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.5511/plantbiotechnology.19.0220b
出版者・発行元
日本植物細胞分子生物学会

<p>Transient protein expression is an effective tool to rapidly unravel novel gene functions, such as transcriptional activity of promoters and sub-cellular localization of proteins. However, transient expression is not applicable to some species and varieties because of insufficient expression levels. We recently developed one of the strongest agroinfiltration-based transient protein expression systems for plant cells, termed 'Tsukuba system.' About 4 mg/g fresh weight of protein expression in Nicotiana benthamiana was obtained using this system. The vector pBYR2HS, which contains a geminiviral replication system and a double terminator, can be used in various plant species and varieties, including lettuces, eggplants, tomatoes, hot peppers, and orchids. In this study, we assessed the applicability of the Tsukuba system to several species of legumes, including Lotus japonicus, soybean Glycine max, and common bean Phaseolus vulgaris. The GFP protein was transiently expressed in the seedpods of all examined legume species, however, protein expression in leaves was observed only in P. vulgaris. Taken together, our system is an effective tool to ex

リンク情報
DOI
https://doi.org/10.5511/plantbiotechnology.19.0220b

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