論文

査読有り
2019年4月

Targeted Base Editing with CRISPR-Deaminase in Tomato

Methods in molecular biology (Clifton, N.J.)
  • Shimatani, Zenpei
  • ,
  • Ariizumi, Tohru
  • ,
  • Fujikura, Ushio
  • ,
  • Kondo, Akihiko
  • ,
  • Ezura, Hiroshi
  • ,
  • Nishida, Keiji

1917
開始ページ
297
終了ページ
307
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/978-1-4939-8991-1_22

The Target-AID system, consisting of a complex of cytidine deaminase and deficient CRISPR/Cas9, enables highly specific genomic nucleotide substitutions without the need for template DNA. The Cas9-fused cytidine deaminase is guided by sgRNAs and catalyzes the conversion of cytosine to uracil. The resulting U-G DNA mismatches trigger nucleotide substitutions (C to T or G to A) through DNA replication and repair pathways. Target-AID also retains the benefits of conventional CRISPR/Cas9 including robustness in various organisms, high targeting efficiency, and multiplex simultaneous gene editing. Our research group recently developed plant-optimized Target-AID system and demonstrated targeted base editing in tomato and rice. In this chapter, we introduce methods for Target-AID application in tomato.

リンク情報
DOI
https://doi.org/10.1007/978-1-4939-8991-1_22

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