論文

査読有り
2017年3月

Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9

SCIENTIFIC REPORTS
  • Risa Ueta
  • ,
  • Chihiro Abe
  • ,
  • Takahito Watanabe
  • ,
  • Shigeo S. Sugano
  • ,
  • Ryosuke Ishihara
  • ,
  • Hiroshi Ezura
  • ,
  • Yuriko Osakabe
  • ,
  • Keishi Osakabe

7
1
開始ページ
507
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-017-00501-4
出版者・発行元
NATURE PUBLISHING GROUP

Parthenocarpy in horticultural crop plants is an important trait with agricultural value for various industrial purposes as well as direct eating quality. Here, we demonstrate a breeding strategy to generate parthenocarpic tomato plants using the CRISPR/Cas9 system. We optimized the CRISPR/Cas9 system to introduce somatic mutations effectively into SlIAA9-a key gene controlling parthenocarpy-with mutation rates of up to 100% in the T0 generation. Furthermore, analysis of off-target mutations using deep sequencing indicated that our customized gRNAs induced no additional mutations in the host genome. Regenerated mutants exhibited morphological changes in leaf shape and seedless fruit-a characteristic of parthenocarpic tomato. And the segregated next generation (T1) also showed a severe phenotype associated with the homozygous mutated genome. The system developed here could be applied to produce parthenocarpic tomato in a wide variety of cultivars, as well as other major horticultural crops, using this precise and rapid breeding technique.

Web of Science ® 被引用回数 : 79

リンク情報
DOI
https://doi.org/10.1038/s41598-017-00501-4
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000397811900002&DestApp=WOS_CPL

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