論文

査読有り
2012年6月

Availability of Micro-Tom mutant library combined with TILLING in molecular breeding of tomato fruit shelf-life

BREEDING SCIENCE
  • Yoshihiro Okabe
  • ,
  • Erika Asamizu
  • ,
  • Tohru Ariizumi
  • ,
  • Kenta Shirasawa
  • ,
  • Satoshi Tabata
  • ,
  • Hiroshi Ezura

62
2
開始ページ
202
終了ページ
208
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1270/jsbbs.62.202
出版者・発行元
JAPANESE SOC BREEDING

Novel mutant alleles of an ethylene receptor Solanum lycopersicum ETHYLENE RESPONSE1 (SlETR1) gene, Sletr1-1 and Sletr1-2, were isolated from the Micro-Tom mutant library by TILLING in our previous study. They displayed different levels of impaired fruit ripening phenotype, suggesting that these alleles could be a valuable breeding material for improving shelf life of tomato fruit. To conduct practical use of the Sletr1 alleles in tomato breeding, genetic complementation analysis by transformation of genes carrying each allele is required. In this study, we generated and characterized transgenic lines over-expressing Sletr1-1 and Sletr1-2. All transgenic lines displayed ethylene insensitive phenotype and ripening inhibition, indicating that Sletr1-1 and Sletr1-2 associate with the ethylene insensitive phenotype. The level of ethylene sensitivity in the seedling was different between Sletr1-1 and Sletr1-2 transgenic lines, whereas no apparent difference was observed in fruit ripening phenotype. These results suggested that it is difficult to fine-tune the extent of ripening by transgenic approach even if the weaker allele (Sletr1-2) was used. Our present and previous studies indicate that the Micro-Tom mutant library combined with TILLING could be an efficient tool for exploring genetic variations of important agronomic traits in tomato breeding.

Web of Science ® 被引用回数 : 13

リンク情報
DOI
https://doi.org/10.1270/jsbbs.62.202
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000307991000012&DestApp=WOS_CPL

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