論文

査読有り
2011年12月

Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter

TRANSGENIC RESEARCH
  • Tadayoshi Hirai
  • ,
  • You-Wang Kim
  • ,
  • Kazuhisa Kato
  • ,
  • Kyoko Hiwasa-Tanase
  • ,
  • Hiroshi Ezura

20
6
開始ページ
1285
終了ページ
1292
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s11248-011-9495-9
出版者・発行元
SPRINGER

The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene driven by these promoters. Recombinant miraculin protein predominantly accumulated in transgenic tomato lines using the E8 promoter (E8-MIR) only at the red fruit stage. The accumulations were almost uniform among all fruit tissues. When the 35S promoter (35S-MIR) was used, miraculin accumulation in the exocarp was much higher than in other tissues, indicating that the miraculin accumulation pattern can be regulated by using different types of promoters. We also discuss the potential of the E8-MIR lines for practical use.

Web of Science ® 被引用回数 : 8

リンク情報
DOI
https://doi.org/10.1007/s11248-011-9495-9
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000296787200010&DestApp=WOS_CPL

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