論文

査読有り
2011年11月

Tomato TILLING Technology: Development of a Reverse Genetics Tool for the Efficient Isolation of Mutants from Micro-Tom Mutant Libraries

PLANT AND CELL PHYSIOLOGY
  • Yoshihiro Okabe
  • ,
  • Erika Asamizu
  • ,
  • Takeshi Saito
  • ,
  • Chiaki Matsukura
  • ,
  • Tohru Ariizumi
  • ,
  • Cecile Bres
  • ,
  • Christophe Rothan
  • ,
  • Tsuyoshi Mizoguchi
  • ,
  • Hiroshi Ezura

52
11
開始ページ
1994
終了ページ
2005
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/pcp/pcr134
出版者・発行元
OXFORD UNIV PRESS

To accelerate functional genomic research in tomato, we developed a Micro-Tom TILLING (Targeting Induced Local Lesions In Genomes) platform. DNA pools were constructed from 3,052 ethyl methanesulfonate (EMS) mutant lines treated with 0.5 or 1.0% EMS. The mutation frequency was calculated by screening 10 genes. The 0.5% EMS population had a mild mutation frequency of one mutation per 1,710 kb, whereas the 1.0% EMS population had a frequency of one mutation per 737 kb, a frequency suitable for producing an allelic series of mutations in the target genes. The overall mutation frequency was one mutation per 1,237 kb, which affected an average of three alleles per kilobase screened. To assess whether a Micro-Tom TILLING platform could be used for efficient mutant isolation, six ethylene receptor genes in tomato (SlETR1-SlETR6) were screened. Two allelic mutants of SlETR1 (Sletr1-1 and Sletr1-2) that resulted in reduced ethylene responses were identified, indicating that our Micro-Tom TILLING platform provides a powerful tool for the rapid detection of mutations in an EMS mutant library. This work provides a practical and publicly accessible tool for the study of fruit biology and for obtaining novel genetic material that can be used to improve important agronomic traits in tomato.

Web of Science ® 被引用回数 : 112

リンク情報
DOI
https://doi.org/10.1093/pcp/pcr134
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000297046600009&DestApp=WOS_CPL

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