論文

査読有り
2011年12月

Ubiquitin promoter-terminator cassette promotes genetically stable expression of the taste-modifying protein miraculin in transgenic lettuce

PLANT CELL REPORTS
  • Tadayoshi Hirai
  • ,
  • Abdullah Mohammad Shohael
  • ,
  • You-Wang Kim
  • ,
  • Megumu Yano
  • ,
  • Hiroshi Ezura

30
12
開始ページ
2255
終了ページ
2265
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00299-011-1131-x
出版者・発行元
SPRINGER

Lettuce is a commercially important leafy vegetable that is cultivated worldwide, and it is also a target crop for plant factories. In this study, lettuce was selected as an alternative platform for recombinant miraculin production because of its fast growth, agronomic value, and wide availability. The taste-modifying protein miraculin is a glycoprotein extracted from the red berries of the West African native shrub Richadella dulcifica. Because of its limited natural availability, many attempts have been made to produce this protein in suitable alternative hosts. We produced transgenic lettuce with miraculin gene driven either by the ubiquitin promoter/terminator cassette from lettuce or a 35S promoter/nos terminator cassette. Miraculin gene expression and miraculin accumulation in both cassettes were compared by quantitative real-time PCR analysis, Western blotting, and enzyme-linked immunosorbent assay. The expression level of the miraculin gene and protein in transgenic lettuce was higher and more genetically stable in the ubiquitin promoter/terminator cassette than in the 35S promoter/nos terminator cassette. These results demonstrated that the ubiquitin promoter/terminator cassette is an efficient platform for the genetically stable expression of the miraculin protein in lettuce and hence this platform is of benefit for recombinant miraculin production on a commercial scale.

Web of Science ® 被引用回数 : 12

リンク情報
DOI
https://doi.org/10.1007/s00299-011-1131-x
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000297152400010&DestApp=WOS_CPL

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