論文

査読有り
2011年9月

The HSP Terminator of Arabidopsis thaliana Induces a High Level of Miraculin Accumulation in Transgenic Tomatoes

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
  • Tadayoshi Hirai
  • ,
  • Natsuko Kurokawa
  • ,
  • Narendra Duhita
  • ,
  • Kyoko Hiwasa-Tanase
  • ,
  • Kazuhisa Kato
  • ,
  • Ko Kato
  • ,
  • Hiroshi Ezura

59
18
開始ページ
9942
終了ページ
9949
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/jf202501e
出版者・発行元
AMER CHEMICAL SOC

High-level accumulation of the target recombinant protein is a significant issue in heterologous protein expression using transgenic plants. Miraculin, a taste modifying protein, was accumulated in transgenic tomatoes using an expression cassette in which the miraculin gene was expressed by the cauliflower mosaic virus (CaMV) 35S promoter and the heat shock protein (HSP) terminator (MIR-HSP). The HSP terminator was derived from heat shock protein 18.2 in Arabidopsis thaliana. Using this HSP-containing cassette, the miraculin concentration in To transgenic tomato lines was 1.4-13.9% of the total soluble protein (TSP), and that in the T-1 transgenic tomato line homozygous for the miraculin gene reached 17.1% of the TSP. The accumulation level of the target protein was comparable to levels observed with chloroplast transformation. The high-level accumulation of miraculin in To transgenic tomato lines achieved by the HSP terminator was maintained in the successive T-1 generation, demonstrating the genetic stability of this accumulation system.

Web of Science ® 被引用回数 : 18

リンク情報
DOI
https://doi.org/10.1021/jf202501e
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000295059100028&DestApp=WOS_CPL

エクスポート
BibTeX RIS