論文

査読有り
2011年2月

TOMATOMA: A Novel Tomato Mutant Database Distributing Micro-Tom Mutant Collections

PLANT AND CELL PHYSIOLOGY
  • Takeshi Saito
  • ,
  • Tohru Ariizumi
  • ,
  • Yoshihiro Okabe
  • ,
  • Erika Asamizu
  • ,
  • Kyoko Hiwasa-Tanase
  • ,
  • Naoya Fukuda
  • ,
  • Tsuyoshi Mizoguchi
  • ,
  • Yukiko Yamazaki
  • ,
  • Koh Aoki
  • ,
  • Hiroshi Ezura

52
2
開始ページ
283
終了ページ
296
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/pcp/pcr004
出版者・発行元
OXFORD UNIV PRESS

The tomato is an excellent model for studies of plants bearing berry-type fruits and for experimental studies of the Solanaceae family of plants due to its conserved genetic organization. In this study, a comprehensive mutant tomato population was generated in the background of Micro-Tom, a dwarf, rapid-growth variety. In this and previous studies, a family including 8,598 and 6,422 M-2 mutagenized lines was produced by ethylmethane sulfonate (EMS) mutagenesis and gamma-ray irradiation, and this study developed and investigated these M-2 plants for alteration of visible phenotypes. A total of 9,183 independent M-2 families comprising 91,830 M-2 plants were inspected for phenotypic alteration, and 1,048 individual mutants were isolated. Subsequently, the observed mutant phenotypes were classified into 15 major categories and 48 subcategories. Overall, 1,819 phenotypic categories were found in 1,048 mutants. Of these mutants, 549 were pleiotropic, whereas 499 were non-pleiotropic. Multiple different mutant alleles per locus were found in the mutant libraries, suggesting that the mutagenized populations were nearly saturated. Additionally, genetic analysis of backcrosses indicated the successful inheritance of the mutations in BC1F2 populations, confirming the reproducibility in the morphological phenotyping of the M-2 plants. To integrate and manage the visible phenotypes of mutants and other associated data, we developed the in silico database TOMATOMA, a relational system interfacing modules between mutant line names and phenotypic categories. TOMATOMA is a freely accessible database, and these mutant recourses are available through the TOMATOMA (http://tomatoma.nbrp.jp/index.jsp).

リンク情報
DOI
https://doi.org/10.1093/pcp/pcr004
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000287254000010&DestApp=WOS_CPL
ID情報
  • DOI : 10.1093/pcp/pcr004
  • ISSN : 0032-0781
  • Web of Science ID : WOS:000287254000010

エクスポート
BibTeX RIS