論文

査読有り
2019年12月25日

Multiplex exome sequencing reveals genome-wide frequency and distribution of mutations in the ‘Micro-Tom’ Targeting Induced Local Lesions in Genomes (TILLING) mutant library

Plant Biotechnology
  • Yano, Ryoichi
  • ,
  • Hoshikawa, Ken
  • ,
  • Okabe, Yoshi
  • ,
  • ning, Wang
  • ,
  • Dung, Pham Thi
  • ,
  • Imriani, Pulungan Sri
  • ,
  • Shiba, Hiroshi
  • ,
  • Ariizumi, Tohru
  • ,
  • Ezura, Hiroshi

36
4
開始ページ
223
終了ページ
231
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.5511/plantbiotechnology.19.0830a
出版者・発行元
JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY

While the ‘Micro-Tom’ TILLING mutant library is used for a wide range of purposes, including both basic research of gene function and breeding of commercial cultivars, genome-wide distribution and frequency of mutations have not yet been thoroughly elucidated on a population scale. In this study, we developed a 96-plex exome sequencing method to identify and analyze mutations within the TILLING mutants that were developed in the University of Tsukuba. First, an Illumina paired-end sequencing coupled with 96-plex exome capture resulted in the acquisition of an exome sequence dataset with an average read count of 5.6 million for the 95 mutants. Over 98% of the capture target region could be covered by the short reads with an averaged read depth of 12.8, which enabled us to identify single nucleotide polymorphisms and Indels in a genome-wide manner. By subtracting intra-cultivar DNA variations that are present between wild-type ‘Micro-Tom’ lines, we identified 241,391 mutation candidates in 95 mutant individuals. Of these, 64,319 and 6,480 mutations were expected to cause protein amino acid substitutions or premature stop codon, respectively. Based on the exome mutation datase

リンク情報
DOI
https://doi.org/10.5511/plantbiotechnology.19.0830a
URL
https://www.jstage.jst.go.jp/article/plantbiotechnology/36/4/36_19.0830a/_pdf

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