論文

2019年10月

Super-Agrobacterium ver. 4: Improving the Transformation Frequencies and Genetic Engineering Possibilities for Crop Plants

Frontiers in plant science
  • Nonaka, Satoko
  • ,
  • Someya, Tatsuhiko
  • ,
  • Kadota, Yasuhiro
  • ,
  • Nakamura, Kouji
  • ,
  • Ezura, Hiroshi

10
開始ページ
1204
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3389/fpls.2019.01204
出版者・発行元
FRONTIERS MEDIA SA

has been utilized for both transient and stable transformations of plants. These transformation methods have been used in fields such as breeding GM crops, protein production in plant cells, and the functional analysis of genes. However, some plants have significantly lower transient gene transfer and stable transformation rates, creating a technical barrier that needs to be resolved. In this study, Super- was updated to ver. 4 by introducing both the ACC deaminase () and GABA transaminase () genes, whose resultant enzymes degrade ACC, the ethylene precursor, and GABA, respectively. strain GV2260, which is similar to other major strains (EHA105, GV3101, LBA4404, and MP90), was used in this study. The abilities of the Super- ver. 4 were evaluated in , "Micro-Tom," , and . Super- ver. 4 showed the highest T-DNA transfer (transient transformation) frequencies in and , but not in and . In tomato, Super- ver. 4 increased the stable transformation rate by 3.6-fold compared to the original GV2260 strain. Super- ver. 4 enables reduction of the amount of time and labor required for transformations by approximately 72%, and is therefore a more effective and powerful tool for plant geneti

リンク情報
DOI
https://doi.org/10.3389/fpls.2019.01204

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