論文

2020年9月

Fruit setting rewires central metabolism via gibberellin cascades

Proceedings of the National Academy of Sciences of the United States of America
  • Shinozaki, Yoshihito
  • ,
  • Beauvoit, Bertrand P
  • ,
  • Takahara, Masaru
  • ,
  • Hao, Shuhei
  • ,
  • Ezura, Kentaro
  • ,
  • Andrieu, Marie-Hélène
  • ,
  • Nishida, Keiji
  • ,
  • Mori, Kazuki
  • ,
  • Suzuki, Yutaka
  • ,
  • Kuhara, Satoshi
  • ,
  • Enomoto, Hirofumi
  • ,
  • Kusano, Miyako
  • ,
  • Fukushima, Atsushi
  • ,
  • Mori, Tetsuya
  • ,
  • Kojima, Mikiko
  • ,
  • Kobayashi, Makoto
  • ,
  • Sakakibara, Hitoshi
  • ,
  • Saito, Kazuki
  • ,
  • Ohtani, Yuya
  • ,
  • Bénard, Camille
  • ,
  • Prodhomme, Duyen
  • ,
  • Gibon, Yves
  • ,
  • Ezura, Hiroshi
  • ,
  • Ariizumi, Tohru

117
38
開始ページ
23970
終了ページ
23981
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1073/pnas.2011859117

Fruit set is the process whereby ovaries develop into fruits after pollination and fertilization. The process is induced by the phytohormone gibberellin (GA) in tomatoes, as determined by the constitutive GA response mutant However, the role of GA on the metabolic behavior in fruit-setting ovaries remains largely unknown. This study explored the biochemical mechanisms of fruit set using a network analysis of integrated transcriptome, proteome, metabolome, and enzyme activity data. Our results revealed that fruit set involves the activation of central carbon metabolism, with increased hexoses, hexose phosphates, and downstream metabolites, including intermediates and derivatives of glycolysis, the tricarboxylic acid cycle, and associated organic and amino acids. The network analysis also identified the transcriptional hub gene , that coordinated metabolic activation. Furthermore, a kinetic model of sucrose metabolism predicted that the sucrose cycle had high activity levels in unpollinated ovaries, whereas it was shut down when sugars rapidly accumulated in vacuoles in fruit-setting ovaries, in a time-dependent manner via tonoplastic sugar carriers. Moreover, fruit set at least par

リンク情報
DOI
https://doi.org/10.1073/pnas.2011859117

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