2006年5月
Efficient DNA alkylation by a pyrrole-imidazole CBI conjugate with an indole linker: Sequence-specific alkylation with nine-base-pair recognition
BIOCONJUGATE CHEMISTRY
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- 巻
- 17
- 号
- 3
- 開始ページ
- 715
- 終了ページ
- 720
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/bc060022w
- 出版者・発行元
- AMER CHEMICAL SOC
Conjugates 7, 8, and 10 of N- methylpyrrole ( Py)- N- methylimidazole ( Im) polyamides and 1,2,9,9a- tetrahydrocyclopropa[ 1,2- c] benz[ 1,2- e] indol- 4- one ( CBI) with a 5- amino- 1H- indole- 2- carbonyl linker were synthesized by Fmoc solid- phase synthesis and a subsequent liquid- phase coupling procedure. The DNA alkylating abilities of conjugates 7, 8, 6b, and 10 were examined using Texas Red- labeled PCR fragments and high- resolution denaturing gel electrophoresis. CBI conjugates 7 and 8 exhibited highly efficient sequence- specific DNA alkylation comparable with previous CBI conjugates with a vinyl linker. In particular, conjugate 10, with a 10- ringed hairpin Py- Im polyamide, alkylated at the adenine of 5 - ACAAATCCA- 3. Introduction of an indole linker greatly facilitated the synthesis of sequence- specific alkylating Py- Im polyamides.
- リンク情報
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- DOI
- https://doi.org/10.1021/bc060022w
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902265474540923
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/16704209
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000237576000019&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1021/bc060022w
- ISSN : 1043-1802
- J-Global ID : 200902265474540923
- PubMed ID : 16704209
- Web of Science ID : WOS:000237576000019