2014年1月
Distinct DNA-based epigenetic switches trigger transcriptional activation of silent genes in human dermal fibroblasts
SCIENTIFIC REPORTS
- 巻
- 4
- 号
- 開始ページ
- 3843
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/srep03843
- 出版者・発行元
- NATURE PUBLISHING GROUP
The influential role of the epigenome in orchestrating genome-wide transcriptional activation instigates the demand for the artificial genetic switches with distinct DNA sequence recognition. Recently, we developed a novel class of epigenetically active small molecules called SAHA-PIPs by conjugating selective DNA binding pyrrole-imidazole polyamides (PIPs) with the histone deacetylase inhibitor SAHA. Screening studies revealed that certain SAHA-PIPs trigger targeted transcriptional activation of pluripotency and germ cell genes in mouse and human fibroblasts, respectively. Through microarray studies and functional analysis, here we demonstrate for the first time the remarkable ability of thirty-two different SAHA-PIPs to trigger the transcriptional activation of exclusive clusters of genes and noncoding RNAs. QRT-PCR validated the microarray data, and some SAHA-PIPs activated therapeutically significant genes like KSR2. Based on the aforementioned results, we propose the potential use of SAHA-PIPs as reagents capable of targeted transcriptional activation.
- リンク情報
- ID情報
-
- DOI : 10.1038/srep03843
- ISSN : 2045-2322
- PubMed ID : 24457603
- Web of Science ID : WOS:000330137900001