2015年1月
Rational design of a high-affinity, fast, red calcium indicator R-CaMP2
NATURE METHODS
- 巻
- 12
- 号
- 1
- 開始ページ
- 64
- 終了ページ
- 70
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/NMETH.3185
- 出版者・発行元
- NATURE PUBLISHING GROUP
Fluorescent Ca2+ reporters are widely used as readouts of neuronal activities. Here we designed R-CaMP2, a high-affinity red genetically encoded calcium indicator (GECI) with a Hill coefficient near 1. Use of the calmodulin-binding sequence of CaMKK-a and CaMKK-beta in lieu of an M13 sequence resulted in threefold faster rise and decay times of Ca2+ transients than R-CaMP1.07. These features allowed resolving single action potentials (APs) and recording fast AP trains up to 20-40 Hz in cortical slices. Somatic and synaptic activities of a cortical neuronal ensemble in vivo were imaged with similar efficacy as with previously reported sensitive green GECIs. Combining green and red GECIs, we successfully achieved dual-color monitoring of neuronal activities of distinct cell types, both in the mouse cortex and in freely moving Caenorhabditis elegans. Dual imaging using R-CaMP2 and green GECIs provides a powerful means to interrogate orthogonal and hierarchical neuronal ensembles in vivo.
- リンク情報
- ID情報
-
- DOI : 10.1038/NMETH.3185
- ISSN : 1548-7091
- eISSN : 1548-7105
- PubMed ID : 25419959
- Web of Science ID : WOS:000347668600021