論文

査読有り 国際誌
2020年8月27日

Comparative Studies of the Fluorescence Properties of Microbial Rhodopsins: Spontaneous Emission Versus Photointermediate Fluorescence.

The journal of physical chemistry. B
  • Keiichi Kojima
  • ,
  • Rika Kurihara
  • ,
  • Masayuki Sakamoto
  • ,
  • Tsukasa Takanashi
  • ,
  • Hikaru Kuramochi
  • ,
  • Xiao Min Zhang
  • ,
  • Haruhiko Bito
  • ,
  • Tahei Tahara
  • ,
  • Yuki Sudo

124
34
開始ページ
7361
終了ページ
7367
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/acs.jpcb.0c06560

Rhodopsins are seven-transmembrane photoreceptor proteins that bind to the retinal chromophore and have been utilized as a genetically encoded voltage indicator (GEVI). So far, archaerhodopsin-3 (AR3) has been successfully used as a GEVI, despite its low fluorescence intensity. We performed comparative and quantitative fluorescence analyses of 15 microbial rhodopsins to explore these highly fluorescent molecules and to clarify their fluorescence mechanism. These rhodopsins showed a wide range of fluorescence intensities in mouse hippocampal neurons. Some of them, GR, HwBR, IaNaR, MR, and NpHR, showed fluorescence intensities comparable with or higher than that of AR3, suggesting their potential for GEVIs. The fluorescence intensity in neurons correlated with that of the bright fluorescent photointermediate such as a Q-intermediate (R = 0.75), suggesting that the fluorescence in neurons originates from the fluorescence of the photointermediate. Our findings provide a crucial step for producing next-generation rhodopsin-based GEVIs.

リンク情報
DOI
https://doi.org/10.1021/acs.jpcb.0c06560
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32790405
ID情報
  • DOI : 10.1021/acs.jpcb.0c06560
  • PubMed ID : 32790405

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