論文

査読有り
2014年5月

The Novel Nuclear Envelope Protein KAKU4 Modulates Nuclear Morphology in Arabidopsis

The Plant Cell
  • Chieko Goto
  • ,
  • Kentaro Tamura
  • ,
  • Yoichiro Fukao
  • ,
  • Tomoo Shimada
  • ,
  • Ikuko Hara-Nishimura

26
5
開始ページ
2143
終了ページ
2155
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1105/tpc.113.122168
出版者・発行元
AMER SOC PLANT BIOLOGISTS

In animals, the nuclear lamina is a fibrillar meshwork on the inner surface of the nuclear envelope, composed of coiled-coil lamin proteins and lamin binding membrane proteins. Plants also have a meshwork on the inner surface of the nuclear envelope, but little is known about its composition other than the presence of members of the CROWDED NUCLEI (CRWN) protein family, possible plant lamin analogs. Here, we describe a candidate lamina component, based on two Arabidopsis thaliana mutants (kaku2 and kaku4) with aberrant nuclear morphology. The responsible gene in kaku2 encodes CRWN1, and the responsible gene in kaku4 encodes a plant-specific protein of unknown function (KAKU4) that physically interacts with CRWN1 and its homolog CRWN4. Immunogold labeling revealed that KAKU4 localizes at the inner nuclear membrane. KAKU4 deforms the nuclear envelope in a dose-dependent manner, in association with nuclear membrane invagination and stack formation. The KAKU4-dependent nuclear envelope deformation was enhanced by overaccumulation of CRWN1, although KAKU4 can deform the nuclear envelope even in the absence of CRWN1 and/or CRWN4. Together, these results suggest that plants have evolved a unique lamina-like structure to modulate nuclear shape and size.

リンク情報
DOI
https://doi.org/10.1105/tpc.113.122168
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24824484
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000338771700029&DestApp=WOS_CPL
ID情報
  • DOI : 10.1105/tpc.113.122168
  • ISSN : 1040-4651
  • eISSN : 1532-298X
  • PubMed ID : 24824484
  • Web of Science ID : WOS:000338771700029

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