論文

査読有り 国際誌
2019年8月27日

PD-L1 Induction by Cancer-Associated Fibroblast-Derived Factors in Lung Adenocarcinoma Cells.

Cancers
  • Chihiro Inoue
  • ,
  • Yasuhiro Miki
  • ,
  • Ryoko Saito
  • ,
  • Shuko Hata
  • ,
  • Jiro Abe
  • ,
  • Ikuro Sato
  • ,
  • Yoshinori Okada
  • ,
  • Hironobu Sasano

11
9
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/cancers11091257

Cancer-associated fibroblasts (CAFs) exert various effects upon biological behaviours of cancer. In this study, we examined the correlation of CAFs with the intra-tumoural immune system in the lung adenocarcinoma microenvironment. We studied 27 and 113 cases of lung adenocarcinoma tentatively as Cohorts 1 and 2, respectively. The patients in Cohort 1 received epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) for recurrent lung adenocarcinoma. -smooth muscle actin (-SMA), a surrogate marker for CAFs, was examined by immunohistochemistry. We then examined the effects of CAFs isolated from lung cancer tissues on programmed death ligand 1 (PD-L1) expression in lung adenocarcinoma cell lines. No significant associations were detected between -SMA status and the ratios of CD8/CD4 and Foxp3/CD8 in Cohort 1. However, -SMA status was significantly associated with PD-L1 status in both Cohorts 1 and 2. Conditioned medium of CAFs significantly induced PD-L1 expression in lung adenocarcinoma cell lines, A549, PC-9, and H1975. Among the cytokines examined by antibody array, C-X-C motif chemokine ligand 2 (CXCL2) increased PD-L1 mRNA expression in these cell lines. CXCL2 is therefore considered to have a potential to induce PD-L1 expression in lung adenocarcinoma cells as a result of an interaction between carcinoma cells and CAFs. These findings did firstly demonstrate that CAFs indirectly influenced tumour immunity through increasing PD-L1 expression in lung adenocarcinoma cells.

リンク情報
DOI
https://doi.org/10.3390/cancers11091257
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31462002
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770125
URL
http://orcid.org/0000-0002-3514-8166
ID情報
  • DOI : 10.3390/cancers11091257
  • ISSN : 2072-6694
  • ORCIDのPut Code : 61158606
  • PubMed ID : 31462002
  • PubMed Central 記事ID : PMC6770125

エクスポート
BibTeX RIS