論文

査読有り 国際誌
2004年7月

Expression profiling-based identification of CO2-responsive genes regulated by CCM1 controlling a carbon-concentrating mechanism in Chlamydomonas reinhardtii.

Plant physiology
  • Kenji Miura
  • Takashi Yamano
  • Satoshi Yoshioka
  • Tsutomu Kohinata
  • Yoshihiro Inoue
  • Fumiya Taniguchi
  • Erika Asamizu
  • Yasukazu Nakamura
  • Satoshi Tabata
  • Katsuyuki T Yamato
  • Kanji Ohyama
  • Hideya Fukuzawa
  • 全て表示

135
3
開始ページ
1595
終了ページ
607
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1104/pp.104.041400

Photosynthetic acclimation to CO2-limiting stress is associated with control of genetic and physiological responses through a signal transduction pathway, followed by integrated monitoring of the environmental changes. Although several CO2-responsive genes have been previously isolated, genome-wide analysis has not been applied to the isolation of CO2-responsive genes that may function as part of a carbon-concentrating mechanism (CCM) in photosynthetic eukaryotes. By comparing expression profiles of cells grown under CO2-rich conditions with those of cells grown under CO2-limiting conditions using a cDNA membrane array containing 10,368 expressed sequence tags, 51 low-CO2 inducible genes and 32 genes repressed by low CO2 whose mRNA levels were changed more than 2.5-fold in Chlamydomonas reinhardtii Dangeard were detected. The fact that the induction of almost all low-CO2 inducible genes was impaired in the ccm1 mutant suggests that CCM1 is a master regulator of CCM through putative low-CO2 signal transduction pathways. Among low-CO2 inducible genes, two novel genes, LciA and LciB, were identified, which may be involved in inorganic carbon transport. Possible functions of low-CO2 inducible and/or CCM1-regulated genes are discussed in relation to the CCM.

リンク情報
DOI
https://doi.org/10.1104/pp.104.041400
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/15235119
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC519074
ID情報
  • DOI : 10.1104/pp.104.041400
  • ISSN : 0032-0889
  • PubMed ID : 15235119
  • PubMed Central 記事ID : PMC519074

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