2017年12月1日
CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs
Scientific Reports
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- 巻
- 7
- 号
- 1
- 開始ページ
- 10487
- 終了ページ
- 10487
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/s41598-017-08596-5
- 出版者・発行元
- NATURE PUBLISHING GROUP
Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.
- リンク情報
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- DOI
- https://doi.org/10.1038/s41598-017-08596-5
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/28874671
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585254
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000409309300075&DestApp=WOS_CPL
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85028880250&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85028880250&origin=inward
- ID情報
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- DOI : 10.1038/s41598-017-08596-5
- ISSN : 2045-2322
- eISSN : 2045-2322
- PubMed ID : 28874671
- PubMed Central 記事ID : PMC5585254
- SCOPUS ID : 85028880250
- Web of Science ID : WOS:000409309300075