論文

査読有り
2014年12月

Comparison of a two-stage system with low pressure carbon dioxide microbubbles and heat treatment on the inactivation of Saccharomyces pastorianus cells

FOOD CONTROL
  • Fumiyuki Kobayashi
  • ,
  • Masaki Sugiura
  • ,
  • Hiromi Ikeura
  • ,
  • Michio Sato
  • ,
  • Sachiko Odake
  • ,
  • Masahiko Tamaki

46
開始ページ
35
終了ページ
40
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.foodcont.2014.04.034
出版者・発行元
ELSEVIER SCI LTD

To clarify how Saccharomyces pastorianus cells were affected by a two-stage system that was heating and pressurizing after microbubbled carbon dioxide (MB-CO2) was mixed with the S. pastorianus suspension at low temperature and pressure (two-stage MB-CO2), S. pastorianus cells were observed by electronic microscopy and stained with propidium iodide (PI). Furthermore, the amounts of nucleic acid and protein leaked from treated S. pastorianus cells were determined and intracellular enzyme activities were measured. It was observed by scanning electric microscopy that wrinkles in S. pastorianus cells treated by two-stage MB-CO2 with a heating coil at 50 degrees C (MB50) and heat treatments at 50 degrees C and 80 degrees C (HSO and H80) were more than those in untreated (NT) cells. Upon observation with transmission electron microscopy, it suggested that MB50 had a direct effect on the intracellular substrate, although little influence on the membrane, whereas H80 cells showed visible damage to cell membranes. However, it was recognized that PI intensity in MB50 cells was great than that in NT, HSO and H80 cells, and that the amount of nucleic acid and protein leaked from H80 cells was significantly higher than that of NT, MB50 and HSO cells. Furthermore, the enzyme inactivation efficiency in MB50 cells was the same as in H80 cells. These results estimate that inactivation of S. pastorianus by two-stage MB-CO2 was due to actions of MB-CO2 on the cell membrane and the intracellular substrate such as enzyme inactivation. (C) 2014 Elsevier Ltd. All rights reserved.

Web of Science ® 被引用回数 : 7

リンク情報
DOI
https://doi.org/10.1016/j.foodcont.2014.04.034
Scopus
https://www.scopus.com/record/display.uri?eid=2-s2.0-84901445522&origin=inward
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000340300300006&DestApp=WOS_CPL
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84901445522&origin=inward