2016年12月
A photoactivatable Cre-loxP recombination system for optogenetic genome engineering
NATURE CHEMICAL BIOLOGY
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- 巻
- 12
- 号
- 12
- 開始ページ
- 1059
- 終了ページ
- +
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/NCHEMBIO.2205
- 出版者・発行元
- NATURE PUBLISHING GROUP
Genome engineering techniques represented by the Cre-loxP recombination system have been used extensively for biomedical research. However, powerful and useful techniques for genome engineering that have high spatiotemporal precision remain elusive. Here we develop a highly efficient photoactivatable Cre recombinase (PA-Cre) to optogenetically control genome engineering in vivo. PA-Cre is based on the reassembly of split Cre fragments by light-inducible dimerization of the Magnet system. PA-Cre enables sharp induction (up to 320-fold) of DNA recombination and is efficiently activated even by low-intensity illumination (similar to 0.04 W m(-2)) or short periods of pulsed illumination (similar to 30 s). We demonstrate that PA-Cre allows for efficient DNA recombination in an internal organ of living mice through noninvasive external illumination using a LED light source. The present PA-Cre provides a powerful tool to greatly facilitate optogenetic genome engineering in vivo.
- リンク情報
- ID情報
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- DOI : 10.1038/NCHEMBIO.2205
- ISSN : 1552-4450
- eISSN : 1552-4469
- PubMed ID : 27723747
- Web of Science ID : WOS:000388582900015