2017年11月
Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1
NUCLEIC ACIDS RESEARCH
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- 巻
- 45
- 号
- 20
- 開始ページ
- e172
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/nar/gkx804
- 出版者・発行元
- OXFORD UNIV PRESS
Light-inducible systems allow spatiotemporal control of a variety of biological activities. Here, we report newly optimized optogenetic tools to induce transcription with light in mammalian cells, using the Arabidopsis photoreceptor Flavin Kelch-repeat F-box 1 (FKF1) and its binding partner GIGANTEA (Gl) as well as CRY2/CIB1. By combining the mutagenesis of FKF1 with the optimization of a split FKF1/GI dimerized Gal4-VP16 transcriptional system, we identified constructs enabling significantly improved light-triggered transcriptional induction. In addition, we have improved the CRY2/CIB1-based light-inducible transcription with split construct optimization. The improvements regarding the FKF1/Gland CRY2/CIB1-based systems will be widely applicable for the light-dependent control of transcription in mammalian cells.
- リンク情報
- ID情報
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- DOI : 10.1093/nar/gkx804
- ISSN : 0305-1048
- eISSN : 1362-4962
- PubMed ID : 29040770
- Web of Science ID : WOS:000416164300006