2014年3月
Genetically Engineered Photoinducible Homodimerization System with Improved Dimer-Forming Efficiency
ACS CHEMICAL BIOLOGY
- ,
- ,
- ,
- 巻
- 9
- 号
- 3
- 開始ページ
- 617
- 終了ページ
- 621
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/cb400836k
- 出版者・発行元
- AMER CHEMICAL SOC
Vivid (VVD) is a photoreceptor derived from Neurospora Crassa that rapidly forms a homodimer in response to blue light. Although VVD has several advantages over other photoreceptors as photoinducible homodimerization system, VVD has a critical limitation in its low dimer-forming efficiency. To overcome this limitation of wild-type VVD, here we conduct site-directed saturation mutagenesis in the homodimer interface of VVD. We have found that the Ile52Cys mutation of VVD (VVD-52C) substantially improves its homodimer-forming efficiency up to 180%. We have demonstrated the utility of VVD-52C for making a light-inducible gene expression system more robust. In addition, using VVD-52C, we have developed photoactivatable caspase-9, which enables optical control of apoptosis of mammalian cells. The present genetically engineered photoinducible homodimerization system can provide a powerful tool to optically control a broad range of molecular processes in the cell.
- リンク情報
- ID情報
-
- DOI : 10.1021/cb400836k
- ISSN : 1554-8929
- eISSN : 1554-8937
- PubMed ID : 24428544
- Web of Science ID : WOS:000333477300006