The invasion of alien species of freshwater invertebrates and the associated changes in the faunistic composi- tion of local ecosystems are a serious threat to the conservation of biodiversity. In this work, we supplement the existing list of alien species with information about three more species of non-native freshwater invertebrates, which have formed stable populations in water bodies of Ukraine. These are two representatives of flatworms: Girardia tigrina (Girard, 1850) and Stenostomum saliens Kepner & Carter, 1931, as well as a representative of copepods, Eucyclops roseus Ishida, 1997.