2016年2月
Regulation of the chitin degradation and utilization system by the ChiX small RNA in Serratia marcescens 2170
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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- 巻
- 80
- 号
- 2
- 開始ページ
- 376
- 終了ページ
- 385
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1080/09168451.2015.1083399
- 出版者・発行元
- TAYLOR & FRANCIS LTD
Serratia marcescens 2170 produces three different types of chitinases and chitin-binding protein CBP21. We found that transposon insertion into the 5 untranslated region (5 UTR) of chiPQ-ctb led to defective chitinase and CBP21 production. ChiX small RNA possessed the complementary sequence of the 5 UTRs of the chiPQ-ctb and chiR and repressed the expression of chiP and chiR. ChiX was detected in a medium containing glucose, glycerol, GlcNAc, and (GlcNAc)(2), but the expression of both chiP and chiR was only observed in a medium containing (GlcNAc)(2). chiX mutant produced chitinases, CBP21, and chitobiase without induction. chiP transcripts were more abundant than those of chiR or chiX in a medium containing (GlcNAc)(2). These results suggest that the constitutively expressed ChiX binds to the highly abundant chiP 5 UTR, thereby leading to the induction of chiR mRNA translation and the subsequent expression of chitinases and CBP21.
- リンク情報
- ID情報
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- DOI : 10.1080/09168451.2015.1083399
- ISSN : 0916-8451
- eISSN : 1347-6947
- PubMed ID : 26364670
- Web of Science ID : WOS:000367835500023