Papers

Peer-reviewed
2014

Identification of a Csr system in Serratia marcescens 2170

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
  • Manabu Ito
  • ,
  • Kazuki Nomura
  • ,
  • Hayuki Sugimoto
  • ,
  • Takeshi Watanabe
  • ,
  • Kazushi Suzuki

Volume
60
Number
2
First page
79
Last page
88
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.2323/jgam.60.79
Publisher
MICROBIOL RES FOUNDATION

The carbon storage regulator (Csr) global regulatory system is conserved in many eubacteria and coordinates the expression of various genes that facilitate adaptation during the major physiological growth phase. The Csr system in Escherichia coli comprises an RNA-binding protein, CsrA; small non-coding RNAs, CsrB and CsrC; and a decay factor for small RNAs, CsrD. In this study, we identified the Csr system in Serratia marcescens 2170. S. marcescens CsrA was 97% identical to E. coli CsrA. CsrB and CsrC RNAs had typical stem-loop structures, including a GGA motif that is the CsrA binding site. CsrD was composed of N-terminal two times transmembrane region and HAMP-like, GGDEF, and EAL domains. Overexpression of S. marcescens csr genes complemented the phenotype of E. coli csr mutants. S. marcescens CsrD affected the decay of CsrB and CsrC RNAs in E. coli. These results suggest that the Csr system in S. marcescens is composed of an RNA-binding protein, two Csr small RNAs, and a decay factor for Csr small RNAs.

Link information
DOI
https://doi.org/10.2323/jgam.60.79
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24859866
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000336674000005&DestApp=WOS_CPL
ID information
  • DOI : 10.2323/jgam.60.79
  • ISSN : 0022-1260
  • eISSN : 1349-8037
  • Pubmed ID : 24859866
  • Web of Science ID : WOS:000336674000005

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