2020年9月1日
Chitinase system of Aeromonas salmonicida, and characterization of enzymes involved in chitin degradation
Bioscience, Biotechnology, and Biochemistry
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- 巻
- 84
- 号
- 9
- 開始ページ
- 1936
- 終了ページ
- 1947
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1080/09168451.2020.1771539
- 出版者・発行元
- Informa UK Limited
The genes encoding chitin-degrading enzymes in Aeromonas salmonicida SWSY-1.411 were identified and cloned in Escherichia coli. The strain contained two glycoside hydrolase (GH) families 18 chitinases: AsChiA and AsChiB, two GH19 chitinases: AsChiC and AsChiD, and an auxiliary activities family 10 protein, lytic polysaccharide monooxygenase: AsLPMO10A. These enzymes were successfully expressed in E. coli and purified. AsChiB had the highest hydrolytic activity against insoluble chitin. AsChiD had the highest activity against water-soluble chitin. The peroxygenase activity of AsLPMO10A was lower compared to SmLPMO10A from Serratia marcescens. Synergism on powdered chitin degradation was observed when AsChiA and AsLPMO10A were combined with other chitinases of this strain. More than twice the increase of the synergistic effect was observed when powdered chitin was treated by a combination of AsLPMO10A with all chitinases. GH19 chitinases suppressed the hyphal growth of Trichoderma reesei.
- リンク情報
- ID情報
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- DOI : 10.1080/09168451.2020.1771539
- ISSN : 0916-8451
- eISSN : 1347-6947
- PubMed ID : 32471324