論文

査読有り
2004年2月

Cryptogein-induced initial events in tobacco BY-2 cells: Pharmacological characterization of molecular relationship among cytosolic Ca2+ transients, anion efflux and production of reactive oxygen species

PLANT AND CELL PHYSIOLOGY
  • Y Kadota
  • ,
  • T Goh
  • ,
  • H Tomatsu
  • ,
  • R Tamauchi
  • ,
  • K Higashi
  • ,
  • S Muto
  • ,
  • K Kuchitsu

45
2
開始ページ
160
終了ページ
170
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/pcp/pch020
出版者・発行元
OXFORD UNIV PRESS

Ion fluxes and the production of reactive oxygen species (ROS) are early events that follow elicitor treatment or microbial infection. However, molecular mechanisms for these responses as well as their relationship have been controversial and still largely unknown. We here simultaneously monitored the temporal sequence of initial events at the plasma membrane in suspension-cultured tobacco cells (cell line BY-2) in response to a purified proteinaceous elicitor, cryptogein, which induced hypersensitive cell death. The elicitor induced transient rise in cytosolic Ca2+ concentration ([Ca2+](cyt)) showing two distinct peaks, followed by biphasic (rapid/transient and slow/prolonged) Cl- efflux and H+ influx. Pharmacological analyses suggested that the ;two phases of the [Ca2+](cyt) response correspond to Ca2+ influx through the plasma membrane and an inositol 1,4,5-trisphophate-mediated release of Ca2+ from intracellular Ca2+ stores, respectively, and the [Ca2+](cyt) transients and the Cl- efflux were mutually dependent events regulated by protein phosphorylation. The elicitor also induced production of ROS including O-.(2)- and H2O2, which initiated after the [Ca2+](cyt) rise and required Ca2+ influx, Cl- efflux and protein phosphorylation. An inhibitor of NADPH oxidase, diphenylene iodonium, completely inhibited the elicitor-induced production of O-.(2)- and H2O2, but did not affect the [Ca2+](cyt) transients. These results suggest that cryptogein-induced plasma membrane Ca2+ influx is independent of ROS, and NADPH oxidase dependent ROS production is regulated by these series of ion fluxes.

リンク情報
DOI
https://doi.org/10.1093/pcp/pch020
CiNii Articles
http://ci.nii.ac.jp/naid/10012095745
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/14988486
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000189238700005&DestApp=WOS_CPL
ID情報
  • DOI : 10.1093/pcp/pch020
  • ISSN : 0032-0781
  • CiNii Articles ID : 10012095745
  • PubMed ID : 14988486
  • Web of Science ID : WOS:000189238700005

エクスポート
BibTeX RIS