論文

査読有り
2017年6月

Monitoring and visualizing microRNA dynamics during live cell differentiation using microRNA-responsive non-viral reporter vectors

BIOMATERIALS
  • Hideyuki Nakanishi
  • ,
  • Kenji Miki
  • ,
  • Kaoru R. Komatsu
  • ,
  • Masayuki Umeda
  • ,
  • Megumi Mochizuki
  • ,
  • Azusa Inagaki
  • ,
  • Yoshinori Yoshida
  • ,
  • Hirohide Saito

128
開始ページ
121
終了ページ
135
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.biomaterials.2017.02.033
出版者・発行元
ELSEVIER SCI LTD

MicroRNA (miRNA) activity differs with cell type, suggesting it can be used as a cell marker. In this study, we developed novel miRNA responsive non-viral reporter vectors to continuously monitor and visualize miRNA dynamics during differentiation and to efficiently purify target living cells. Each vector codes miRNA-responsive and reference reporter genes in a single mRNA. These two genes are independent modules but transcribed by a single promoter, which enables us to distinguish miRNA-mediated post transcriptional repression from transcriptional repression. We generated stable, miRNA-responsive vector-containing human induced pluripotent stem cells (hiPSCs) using the piggyBac transposon or episomal vectors. We could continuously monitor the differentiation status of living hiPSCs by detecting the activity of hiPSC-specific miRNA (miR-302a*). In addition, we could selectively sort hiPSC-derived cardiomyocytes using cardiomyocyte-specific miRNA (miR-208a or miR-1)-reporter vectors. Our miRNA reporter system provides a simple way to quantitatively and continuously monitor and visualize changes in the cellular state and should facilitate a broad range of studies that depend on cellular changes including drug discovery and cell-fate conversion. (C) 2017 Elsevier Ltd. All rights reserved.

Web of Science ® 被引用回数 : 10

リンク情報
DOI
https://doi.org/10.1016/j.biomaterials.2017.02.033
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28325684
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000399512800011&DestApp=WOS_CPL

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