論文

査読有り
2010年4月

piggyBac Transposon-mediated Long-term Gene Expression in Mice

MOLECULAR THERAPY
  • Hideyuki Nakanishi
  • ,
  • Yuriko Higuchi
  • ,
  • Shigeru Kawakami
  • ,
  • Fumiyoshi Yamashita
  • ,
  • Mitsuru Hashida

18
4
開始ページ
707
終了ページ
714
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/mt.2009.302
出版者・発行元
CELL PRESS

Transposons are promising systems for somatic gene integration because they can not only integrate exogenous genes efficiently, but also be delivered to a variety of organs using a range of transfection methods. piggyBac (PB) transposon has a high transposability in mammalian cells in vitro, and has been used for genetic and preclinical studies. However, the transposability of PB in mammalian somatic cells in vivo has not been demonstrated yet. Here, we demonstrated PB-mediated sustained gene expression in adult mice. We constructed PB-based plasmid DNA (pDNA) containing reporter [firefly and Gaussia luciferase (Gluc)] genes. Mice were transfected by injection of these pDNAs using a hydrodynamics-based procedure, and the conditions for high-level sustained gene expression were examined. Consequently, gene expressions were sustained over 2 months. Our results suggest that PB is useful for organ-selective somatic integration and sustained gene expression in mammals, and will contribute to basic genetic studies and gene therapies.

Web of Science ® 被引用回数 : 66

リンク情報
DOI
https://doi.org/10.1038/mt.2009.302
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/20104210
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000276636800009&DestApp=WOS_CPL

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