論文

査読有り
2011年7月

Comparison of piggyBac transposition efficiency between linear and circular donor vectors in mammalian cells

JOURNAL OF BIOTECHNOLOGY
  • Hideyuki Nakanishi
  • ,
  • Yuriko Higuchi
  • ,
  • Shigeru Kawakami
  • ,
  • Fumiyoshi Yamashita
  • ,
  • Mitsuru Hashida

154
4
開始ページ
205
終了ページ
208
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jbiotec.2011.05.009
出版者・発行元
ELSEVIER SCIENCE BV

The piggyBac transposon has recently attracted attention as a tool for transgene integration in mammalian cells. However, previous studies involving piggyBac investigated only transposition from circular DNA, although some linear DNA vectors are used to transfect mammalian cells. In this study, we compared the transposition efficiency of piggyBac between linear and circular DNA. Colony counting assay, luciferase assay, and plasmid rescue assay showed that piggyBac transposon can transpose from linear DNA, but its efficiency is lower than the transposition efficiency from circular DNA. These results suggest that circular DNA is more suitable as donor vectors of piggyBac than linear DNA. (C) 2011 Elsevier B. V. All rights reserved.

Web of Science ® 被引用回数 : 6

リンク情報
DOI
https://doi.org/10.1016/j.jbiotec.2011.05.009
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/21640767
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000292958600002&DestApp=WOS_CPL

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