論文

査読有り 国際誌
2020年11月

Immunomodulation Mediated by Azithromycin in Experimental Periapical Inflammation.

Journal of endodontics
  • Ana Cristina Andrada
  • ,
  • Mariane Maffei Azuma
  • ,
  • Hisako Furusho
  • ,
  • Kimito Hirai
  • ,
  • Shuang Xu
  • ,
  • Robert R White
  • ,
  • Hajime Sasaki

46
11
開始ページ
1648
終了ページ
1654
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.joen.2020.07.028

INTRODUCTION: The purpose of the present study was to compare the immunomodulatory effect of azithromycin (AZM), ampicillin (AMP), amoxicillin (AMX), and clindamycin (CLI) in vitro and AZM on preexisting periapical lesions compared with AMP. METHODS: The susceptibility of 4 common human endodontic pathogens (Parvimonas micra, Streptococcus intermedius, Prevotella intermedia, and Fusobacterium nucleatum) to AZM, AMP, AMX, and CLI was confirmed by agar disk diffusion assay. Preexisting periapical lesions in C57BL/6J mice were treated with AZM, AMP, or phosphate-buffered saline (PBS). Periapical bone healing and the pattern of inflammatory cell infiltration were evaluated after a 10-day treatment by micro-computed tomographic and histology, respectively. Besides, the effect of antibiotics in pathogen-stimulated nuclear factor kappa B activation and the production of interleukin 1 alpha and tumor necrosis factor alpha was assessed in vitro by luciferase assay and enzyme-linked immunosorbent assay. RESULTS: All examined endodontic pathogens were susceptible to AZM, AMP, AMX, and CLI. AZM significantly attenuated periapical bone loss versus PBS. PBS resulted in widely diffused infiltration of mixed inflammatory cells. By contrast, AZM brought about localized infiltration of neutrophils and M2 macrophages and advanced fibrosis. Although the effect of AMP on bone was uncertain, inflammatory cell infiltration was considerably milder than PBS. However, most macrophages observed seemed to be M1 macrophages. AZM suppressed pathogen-stimulated nuclear factor kappa B activation and cytokine production, whereas AMP, AMX, and CLI reduced only cytokine production moderately. CONCLUSIONS: This study showed that AZM led to the resolution of preexisting experimental periapical inflammation. Our data provide a perspective on host response in antibiotic selection for endodontic treatment. However, well-designed clinical trials are necessary to better elucidate the benefits of AZM as an adjunctive therapy for endodontic treatment when antibiotic therapy is recommended. Although both AZM and AMP were effective on preexisting periapical lesions, AZM led to advanced wound healing, probably depending on its immunomodulatory effect.

リンク情報
DOI
https://doi.org/10.1016/j.joen.2020.07.028
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32763436
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862415
ID情報
  • DOI : 10.1016/j.joen.2020.07.028
  • PubMed ID : 32763436
  • PubMed Central 記事ID : PMC7862415

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