論文

査読有り
2020年1月

Antimicrobial and antibiofilm effects of abietic acid on cariogenic Streptococcus mutans.

Odontology
  • Yuki Ito
  • ,
  • Takashi Ito
  • ,
  • Keisuke Yamashiro
  • ,
  • Fumi Mineshiba
  • ,
  • Kimito Hirai
  • ,
  • Kazuhiro Omori
  • ,
  • Tadashi Yamamoto
  • ,
  • Shogo Takashiba

108
1
開始ページ
57
終了ページ
65
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s10266-019-00456-0

Dental caries is a type of oral microbiome dysbiosis and biofilm infection that affects oral and systemic conditions. For healthy life expectancy, natural bacteriostatic products are ideal for daily and lifetime use as anti-oral infection agents. This study aimed to evaluate the inhibitory effects of abietic acid, a diterpene derived from pine rosin, on the in vitro growth of cariogenic bacterial species, Streptococcus mutans. The effective minimum inhibitory concentration of abietic acid was determined through observation of S. mutans growth, acidification, and biofilm formation. The inhibitory effects of abietic acid on the bacterial membrane were investigated through the use of in situ viability analysis and scanning electron microscopic analysis. Cytotoxicity of abietic acid was also examined in the context of several human cell lines using tetrazolium reduction assay. Abietic acid was found to inhibit key bacterial growth hallmarks such as colony forming ability, adenosine triphosphate activity (both planktonic and biofilm), acid production, and biofilm formation. Abietic acid was identified as bacteriostatic, and this compound caused minimal damage to the bacterial membrane. This action was different from that of povidone-iodine or cetylpyridinium chloride. Additionally, abietic acid was significantly less cytotoxic compared to povidone-iodine, and it exerted lower toxicity towards epithelial cells and fibroblasts compared to that against monocytic cells. These data suggest that abietic acid may prove useful as an antibacterial and antibiofilm agent for controlling S. mutans infection.

リンク情報
DOI
https://doi.org/10.1007/s10266-019-00456-0
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31520265
ID情報
  • DOI : 10.1007/s10266-019-00456-0
  • PubMed ID : 31520265

エクスポート
BibTeX RIS