Hiroaki Ohishi

Profile Information

Affiliation: Medical Institute of Bioregulation, Kyushu University

Degree: 博士（医学）(九州大学)

Contact information: hohishibioreg.kyushu-u.ac.jp
Researcher number: 70912434
ORCID ID: https://orcid.org/0000-0001-6672-4093
J-GLOBAL ID: 202001018320334180
researchmap Member ID: R000003703

External link: https://bioreg.kyushu-u.ac.jp/labo/ged/

Research History

Oct, 2024 - Present

PRESTO, Japan Science and Technology Agency
Mar, 2023 - Present

Assistant Professor, Kyushu University
May, 2020 - Feb, 2023

Hiroshima University
Apr, 2020 - Apr, 2020

Kyushu University
Apr, 2014 - Mar, 2016

日本学術振興会特別研究員（DC2）, 九州大学

Education

Apr, 2012 - Mar, 2020

Kyushu University
Apr, 2006 - Mar, 2012

Department of Pharmaceutics, Fukuoka University

Papers

Image Analysis Protocol for DNA/RNA/Immunofluorescence (IF)-seqFISH Data.

Hiroaki Ohishi, Hiroshi Ochiai

Methods in molecular biology (Clifton, N.J.), 2856 419-432, 2025 Lead author
Anti-phase clustering of regulatory factors shapes gene bursting

Bitong Li, Yew Yan Wong, Neftali Flores-Rodriguez, Tara Davidson, Matthew S Graus, Valeriia Smialkovska, Hiroaki Ohishi, Angelika Feldmann, Hiroshi Ochiai, Mathias Francois

bioRxiv, Sep 11, 2024
Precise immunofluorescence canceling for highly multiplexed imaging to capture specific cell states

Kosuke Tomimatsu, Takeru Fujii, Ryoma Bise, Kazufumi Hosoda, Yosuke Taniguchi, Hiroshi Ochiai, Hiroaki Ohishi, Kanta Ando, Ryoma Minami, Kaori Tanaka …

Nature Communications, May 8, 2024 Peer-reviewed
Combined and differential roles of ADD domains of DNMT3A and DNMT3L on DNA methylation landscapes in mouse germ cells

Naoki Kubo, Ryuji Uehara, Shuhei Uemura, Hiroaki Ohishi, Kenjiro Shirane, Hiroyuki Sasaki

Nature Communications, 15(1), Apr 16, 2024 Peer-reviewed
Transcription-coupled changes in higher-order genomic structure and transcription hub viscosity prolong enhancer-promoter connectivity

Hiroaki Ohishi, Soya Shinkai, Hitoshi Owada, Takeru Fujii, Kazufumi Hosoda, Shuichi Onami, Takashi Yamamoto, Yasuyuki Ohkawa, Hiroshi Ochiai

bioRxiv, Nov 27, 2023 Lead author
Precise immunofluorescence canceling for highly multiplexed imaging capturing specific cell state

Kosuke Tomimatsu, Takeru Fujii, Ryoma Bise, Kazufumi Hosoda, Yosuke Taniguchi, Hiroshi Ochiai, Hiroaki Ohishi, Kanta Ando, Ryoma Minami, Tachibana Taro …

bioRxiv, Oct 18, 2023
Combined and differential roles of ADD domains of DNMT3A and DNMT3L on DNA methylation landscapes in mouse germ cells

Naoki Kubo, Ryuji Uehara, Shuhei Uemura, Hiroaki Ohishi, Kenjiro Shirane, Hiroyuki Sasaki

bioRxiv, Sep 8, 2023
The DNMT3A ADD domain is required for efficient de novo DNA methylation and maternal imprinting in mouse oocytes

Ryuji Uehara, Wan Kin Au Yeung, Keisuke Toriyama, Hiroaki Ohishi, Naoki Kubo, Hidehiro Toh, Isao Suetake, Kenjiro Shirane, Hiroyuki Sasaki

PLOS Genetics, 19(8) e1010855-e1010855, Aug 1, 2023 Peer-reviewed
Organization of transcription and 3D genome as revealed by live-cell imaging.

Hiroshi Ochiai, Hiroaki Ohishi, Yuko Sato, Hiroshi Kimura

Current opinion in structural biology, 81 102615-102615, May 29, 2023 Peer-reviewed
STREAMING-Tag System: Technology to Enable Visualization of Transcriptional Activity and Subnuclear Localization of Specific Endogenous Genes.

Hiroaki Ohishi, Hiroshi Ochiai

Methods in molecular biology (Clifton, N.J.), 2577 103-122, 2023 Lead author
STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity

Hiroaki Ohishi, Seiru Shimada, Satoshi Uchino, Jieru Li, Yuko Sato, Manabu Shintani, Hitoshi Owada, Yasuyuki Ohkawa, Alexandros Pertsinidis, Takashi Yamamoto …

Nature Communications, Dec 20, 2022 Peer-reviewedLead author
STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity

Hiroaki Ohishi, Seiru Shimada, Satoshi Uchino, Jieru Li, Yuko Sato, Manabu Shintani, Hitoshi Owada, Yasuyuki Ohkawa, Alexandros Pertsinidis, Takashi Yamamoto …

bioRxiv, Jan 6, 2022 Lead author
The DNMT3A PWWP domain is essential for the normal DNA methylation landscape in mouse somatic cells and oocytes.

Kanako Kibe, Kenjiro Shirane, Hiroaki Ohishi, Shuhei Uemura, Hidehiro Toh, Hiroyuki Sasaki

PLoS genetics, 17(5) e1009570, May, 2021 Peer-reviewed
Ddhd1 knockout mouse as a model of locomotive and physiological abnormality in familial spastic paraplegia.

Takuya Morikawa, Hiroaki Ohishi, Kengo Kosaka, Tomofumi Shimojo, Akihiro Nagano, Itsuki Taniguchi, Ryuta Fujioka, Kosei Moriyama, Motoko Unoki, Masatomo Takahashi …

Bioscience reports, 41(2), Feb 12, 2021 Peer-reviewed
Characterization of genetic-origin-dependent monoallelic expression in mouse embryonic stem cells.

Hiroaki Ohishi, Wan Kin Au Yeung, Motoko Unoki, Kenji Ichiyanagi, Kei Fukuda, Shoji Maenohara, Kenjiro Shirane, Hatsune Chiba, Takashi Sado, Hiroyuki Sasaki

Genes to cells : devoted to molecular & cellular mechanisms, 25(1) 54-64, Jan, 2020 Peer-reviewedLead authorCorresponding author
Poorly controlled diabetes during pregnancy and lactation activates the Foxo1 pathway and causes glucose intolerance in adult offspring.

Yukihiro Inoguchi, Kenji Ichiyanagi, Hiroaki Ohishi, Yasutaka Maeda, Noriyuki Sonoda, Yoshihiro Ogawa, Toyoshi Inoguchi, Hiroyuki Sasaki

Scientific reports, 9(1) 10181-10181, Jul 15, 2019 Peer-reviewed
Zfp281 Shapes the Transcriptome of Trophoblast Stem Cells and Is Essential for Placental Development.

Takashi Ishiuchi, Hiroaki Ohishi, Tetsuya Sato, Satoshi Kamimura, Masayoshi Yorino, Shusaku Abe, Atsushi Suzuki, Teruhiko Wakayama, Mikita Suyama, Hiroyuki Sasaki

Cell reports, 27(6) 1742-1754, May 7, 2019 Peer-reviewed
Role of UHRF1 in de novo DNA methylation in oocytes and maintenance methylation in preimplantation embryos.

Shoji Maenohara, Motoko Unoki, Hidehiro Toh, Hiroaki Ohishi, Jafar Sharif, Haruhiko Koseki, Hiroyuki Sasaki

PLoS genetics, 13(10) e1007042, Oct, 2017 Peer-reviewed
Potential role for S100A4 in the disruption of the blood-brain barrier in collagen-induced arthritic mice, an animal model of rheumatoid arthritis.

T Nishioku, K Furusho, A Tomita, H Ohishi, S Dohgu, H Shuto, A Yamauchi, Y Kataoka

Neuroscience, 189 286-92, Aug 25, 2011 Peer-reviewed

Major Misc.

特定RNA分子／DNA領域のライブイメージング技術

大石裕晃, 落合博

生体の科学, 74(3) 260-265, Jun, 2023
【CRISPR最新ツールボックス高効率・高精度なゲノム編集から遺伝子座の制御・検出まであなたの研究を拡張する!】CRISPR-Cas9システムによる特定遺伝子座ライブイメージング

大石裕晃, 落合博

実験医学, 39(8) 1219-1224, May, 2021
【1細胞解析-技術と応用】技術ライブイメージングによる1細胞動態解析

大石裕晃, 落合博

医学のあゆみ, 276(10) 933-939, Mar, 2021
Genomic Imprinting and Epigenome Regulation

34(9) 852-856, 2015

Major Books and Other Publications

イメージングによる空間配置情報を保持した1細胞トランスクリプトーム解析ーseqFISH＋

大石裕晃 (Role: Contributor, 242-246)

羊土社, Dec 18, 2020 (ISBN: 9784758122481)

Awards

Major Presentations

胚性幹細胞における単アリル性発現遺伝子はゲノム及び付随したエピゲノムによって制御される

大石裕晃, 鵜木元香, 一柳健司, 福田渓, 白根健次郎, 前之原章司, 佐渡敬, 佐々木裕之

生命科学系学会合同年次大会, Dec, 2017, 生命科学系学会合同年次大会運営事務局
胚性幹細胞における単アレル性発現遺伝子の由来とその転写制御

大石裕晃, 鵜木元香, 福田渓, 堀恭平, 前之原章司, 千葉初音, 佐渡敬, 佐々木裕之

日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, Dec, 2015, (公社)日本生化学会

Professional Memberships

Research Projects

空間交差型エピゲノムによる超微細な転写操作

戦略的創造研究推進事業（さきがけ）「細胞操作」領域, 科学技術振興機構 (JST), Oct, 2024 - Mar, 2028

大石裕晃
蛍光イメージングによる時空間的なゲノムインプリンティング制御機構の解明

科学研究費助成事業若手研究, 若手研究, 日本学術振興会, Apr, 2022 - Mar, 2025

大石裕晃
膨張顕微鏡法と超解像顕微鏡を組み合わせた高精細クロマチントレーシング技術による卵子エピゲノム形成機構の解明

日本人若手研究者研究助成金, (公財)中島記念国際交流財団, 2024

大石裕晃
新規ＫＲＡＢ－ＺＮＦタンパク質によるエピジェネティック修飾の維持伝達機構の解明

科学研究費助成事業特別研究員奨励費, 特別研究員奨励費, 日本学術振興会, Apr, 2014 - Mar, 2016

大石裕晃
A role of UHRF1 in establishment and maintenance of genomic imprinting

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2012 - Mar, 2015

UNOKI MOTOKO, MAENOHARA Shoji, OHISHI Hiroaki, TANAKA Tomohiro

Profile Information

Research History

Education

Papers

Major Misc.

Major Books and Other Publications

Awards

Major Presentations

Professional Memberships

Research Projects

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