論文

査読有り 責任著者 国際誌
2015年5月

Role of isolated and clustered DNA damage and the post-irradiating repair process in the effects of heavy ion beam irradiation

JOURNAL OF RADIATION RESEARCH
  • Yuka Tokuyama
  • ,
  • Yoshiya Furusawa
  • ,
  • Hiroshi Ide
  • ,
  • Akira Yasui
  • ,
  • Hiroaki Terato

56
3
開始ページ
446
終了ページ
455
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/jrr/rru122
出版者・発行元
OXFORD UNIV PRESS

Clustered DNA damage is a specific type of DNA damage induced by ionizing radiation. Any type of ionizing radiation traverses the target DNA molecule as a beam, inducing damage along its track. Our previous study showed that clustered DNA damage yields decreased with increased linear energy transfer (LET), leading us to investigate the importance of clustered DNA damage in the biological effects of heavy ion beam radiation. In this study, we analyzed the yield of clustered base damage (comprising multiple base lesions) in cultured cells irradiated with various heavy ion beams, and investigated isolated base damage and the repair process in post-irradiation cultured cells. Chinese hamster ovary (CHO) cells were irradiated by carbon, silicon, argon and iron ion beams with LETs of 13, 55, 90 and 200 keV mu m(-1), respectively. Agarose gel electrophoresis of the cells with enzymatic treatments indicated that clustered base damage yields decreased as the LET increased. The aldehyde reactive probe procedure showed that isolated base damage yields in the irradiated cells followed the same pattern. To analyze the cellular base damage process, clustered DNA damage repair was investigated using DNA repair mutant cells. DNA double-strand breaks accumulated in CHO mutant cells lacking Xrcc1 after irradiation, and the cell viability decreased. On the other hand, mouse embryonic fibroblast (Mef) cells lacking both Nth1 and Ogg1 became more resistant than the wild type Mef. Thus, clustered base damage seems to be involved in the expression of heavy ion beam biological effects via the repair process.

リンク情報
DOI
https://doi.org/10.1093/jrr/rru122
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25717060
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4426916
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000356017900006&DestApp=WOS_CPL
URL
http://www.scopus.com/inward/record.url?eid=2-s2.0-84929938447&partnerID=MN8TOARS
ID情報
  • DOI : 10.1093/jrr/rru122
  • ISSN : 0449-3060
  • eISSN : 1349-9157
  • ORCIDのPut Code : 80281208
  • PubMed ID : 25717060
  • PubMed Central 記事ID : PMC4426916
  • SCOPUS ID : 84929938447
  • Web of Science ID : WOS:000356017900006

エクスポート
BibTeX RIS