1997年2月
Replication bypass and mutagenic effect of alpha-deoxyadenosine site-specifically incorporated into single-stranded vectors
NUCLEIC ACIDS RESEARCH
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- 巻
- 25
- 号
- 3
- 開始ページ
- 597
- 終了ページ
- 603
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/nar/25.3.597
- 出版者・発行元
- OXFORD UNIV PRESS
alpha-2'-Deoxyadenosine (alpha) is a major adenine lesion produced by gamma-ray irradiation of DNA under anoxic conditions. In this study, single-stranded recombinant M13 vectors containing alpha were constructed and transfected into Escherichia coli to assess lethal and mutagenic effects of this lesion. The data for a were further compared with those obtained with M13 vectors containing normal A or a model abasic site (F) at the same site. The transfection assay revealed that alpha constituted a moderate block to DNA replication. The in vivo replication capacity to pass through alpha was similar to 20% relative to normal A, but 20-fold higher than that of F constituting an almost absolute replication block. Similar data were obtained by in vitro replication of oligonucleotide templates containing alpha or F by E. coli DNA polymerase I. The mutagenic consequence of replicating M13 DNA containing alpha was analyzed by direct DNA sequencing of progeny phage. Mutagenesis was totally targeted at the site of alpha introduced into the vector. Mutation was exclusively a single nucleotide deletion and no base substitutions were detected. The deletion frequency associated alpha was dependent on the 3'-nearest neighbor base: with the 3'-nearest neighbor base T mutation (deletion) frequency was 26%, whereas 1% with the 3'-nearest neighbor base G. A possible mechanism of the single nucleotide deletion associated with alpha is discussed on the basis of the misinsertion-strand slippage model.
- リンク情報
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- DOI
- https://doi.org/10.1093/nar/25.3.597
- CiNii Articles
- http://ci.nii.ac.jp/naid/80009442625
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/9016601
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1997WG77100021&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1093/nar/25.3.597
- ISSN : 0305-1048
- eISSN : 1362-4962
- CiNii Articles ID : 80009442625
- PubMed ID : 9016601
- Web of Science ID : WOS:A1997WG77100021