論文

査読有り
2000年

Recognition of formamidopyrimidine by Escherichia coli and mammalian thymine glycol glycosylases. Distinctive paired base effects and biological and mechanistic implications

Journal of Biological Chemistry
  • Asagoshi, K.
  • ,
  • Yamada, T.
  • ,
  • Okada, Y.
  • ,
  • Terato, H.
  • ,
  • Ohyama, Y.
  • ,
  • Seki, S.
  • ,
  • Ide, H.

275
32
開始ページ
24781
終了ページ
24786
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1074/jbc.M000576200
出版者・発行元
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

The activity of prokaryotic and mammalian thymine glycol (Tg) glycosylases including Escherichia coli endonuclease III (Endo III) and endonuclease VIII (Endo VIII) and mouse Endo III homologue (mNth1) for formamidopyrimidine (Fapy) has been investigated using defined oligonucleotide substrates, 2,6-Diamino-4-hydroxy-5-N-methylformamidopyrimidine, a methylated Fapy derived from guanine, was site specifically incorporated in the oligonucleotide, The substrates containing Fapy:N pairs (N = A, G, C, T) as well as a Tg:A pair, a physiological substrate of Endo HI, Endo VIII, and mNth1, were treated by the enzymes and nicked products were quantified by gel electrophoresis. The activity of Endo III and Endo VIII for Fapy varied markedly depending on the paired base, being the highest with G (activity relative to Tg = 0.55 (Endo III) and 0.41 (Endo VIII)) and the lowest with C (0.05 (Endo III) and 0.06 (Endo VIII)). In contrast, mNth1 recognized all Fapy pairs equally well and the activity was comparable to Tg, The results obtained in the nicking assay were further substantiated by the analysis of the Schiff base intermediate using NaBH4 trapping assays. These results indicate that Escherichia coli and mammalian Tg glycosylases have a potential activity to recognize Fapy. However, as demonstrated for Fapy:C pairs, their distinctive activities implicate unequal participation in the repair of Fapy lesions in cells.

リンク情報
DOI
https://doi.org/10.1074/jbc.M000576200
CiNii Articles
http://ci.nii.ac.jp/naid/30016328365
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/10827172
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000088683300075&DestApp=WOS_CPL
URL
http://www.scopus.com/inward/record.url?eid=2-s2.0-0034637604&partnerID=MN8TOARS
ID情報
  • DOI : 10.1074/jbc.M000576200
  • ISSN : 0021-9258
  • CiNii Articles ID : 30016328365
  • ORCIDのPut Code : 80281165
  • PubMed ID : 10827172
  • SCOPUS ID : 0034637604
  • Web of Science ID : WOS:000088683300075

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